Springfield is the third-largest city in the state of Missouri and the county seat of Greene County. As of the 2010 census, its population was 159,498. As of 2016, the Census Bureau estimated its population at 167,319. It is one of the two principal cities of the Springfield-Branson Metropolitan Area, which has a population of 541,991 and includes the counties of Christian, Dallas, Greene, Polk, Webster, Stone and Taney. Springfield's nickname is "Queen City of the Ozarks" and it is known as the "Birthplace of Route 66". It is home to several universities, including Missouri State University, Drury University, and Evangel University.